Livestock Research for Rural Development 34 (6) 2022 LRRD Search LRRD Misssion Guide for preparation of papers LRRD Newsletter

Citation of this paper

Effect of replacing dietary protein with cassava leaf meal on performance and nutritional composition of two-spotted crickets (Gryllus bimaculatus)

Vo Lam, Nguyen Thi Bich Hanh and Bui Phan Thu Hang

An Giang University Vietnam National University Ho Chi Minh City, Vietnam
volam.agu@gmail.com

Abstract

This study aimed to evaluate growth performance and chemical composition of crickets fed diets containing increasing proportions of cassava leaf meal as partial replacement of dietary protein. Two-spotted crickets (Gryllus bimaculatus) (n=14,400) with a mean initial weight of 3.1±0.2 mg and seven days of age were housed in cages (300 per cage) and fed diets containing increasing proportions (15, 20, 25, 30, 35, 40 and 45%) of cassava leaf meal replacing soybean and fish meal. The design was a randomized block with 8 treatments and 6 replications (300 crickets per treatment/replicate).

There were curvilinear relationships between levels of cassava leaf meal in the diet and the responses in DM intake, liveweight gain and feed conversion. The feed intake continued to increase up to the highest level of intake of cassava leaf meal but at a reduced rate. In contrast, the response in weight gain and feed conversion showed a negative trend as the level of cassava leaf meal in the diet was increased beyond 20%. The composition of the crickets was affected by cassava leaf meal supplementation with curvilinear increases in protein and ash content but with a curvilinear decrease in lipid content as the level of cassava leaf meal in the diet was increased.

Key words: cassava leaf meal, insects, protein


Introduction

Two thousand and one hundred species of insects are consumed as food in over 110 countries (Jongema 2017). Crickets are the most consumed insects (Magara et al 2019, Orinda 2018, Oonincx et al 2015). Crickets have been consumed as food in Africa, Latin America and Asia (Ayieko et al 2016, Gelfand 1971, Séré et al 2018, Bani 1995, Bodenheimer 1951, Feng et al 2017) and are being appreciated in Australia, America and Europe with the appreciation of their role in food security and for their nutritional benefits (EFSA Scientific Committee 2015, Frigerio et al 2020, Instar Farming 2020, Angie 20197). Their potential is related to their nutritional quality and for being environmentally friendly (with low emission of greenhouse gases), the small land requirement for their production as compared to livestock, and as economically feasible alternatives for the food industry in the future (Van Huis et al 2013, Orinda 2018, Oonincx et al 2015, Halloran et al 2018). Crickets have lower feed conversion rates when compared to livestock (Van Huis et al 2013). Crickets also have the potential to use a wide variety of plant material such as food waste and byproducts from agriculture and the food industry. They are credited cleaning the environment (Magara et al 2019, Orinda 2018, Miech et al 2016, Van Huis and Losasso 2017).

Cassava leaves have potential as feed for crickets in Africa and Asia. Cassava leaf meal provides a high level of protein, is rich in minerals, carotene and vitamin C which can be used for feeding livestock (Anya et al 2019, Li et al 2019, Adedokun et al 2021). Fresh cassava leaves are suitable as feed crickets (Bui Phan Thu Hang et al 2020).

The research described in this paper was designed to evaluate the growth performance and chemical composition of crickets fed diets containing increasing levels of cassava leaf meal in partial substitution of soybean and fish meal. The hypothesis was that cassava leaf meal had potential to be to be a major source of protein in the diet of crickets.


Materials and Methods

Location and source of crickets

This study was carried out in a farm located in Long Xuyen city, An Giang province, Vietnam. The crickets (n = 14 400; 7 days old) were sourced from a rearing unit in An Giang University.

Experimental design

The crickets were fed one of the eight experimental diets. The allocation to treatments was according to a Randomized Block Design with 6 replications (each treatment/replicate had 300 crickets). The experiment lasted for 5 weeks.

Feeding and management

Rice bran, maize, soybean meal and fish meal were bought from the local market. Cassava leaves with petioles and stems (Photo1) were collected from sweet cassava plants cultivated in fields around Long Xuyen city. They were dried in an oven at 55-60 oC for 72 h . All ingredients used in the diets were previously ground fine (Photo 2).

Eight diets were formulated to contain a similar crude protein content (Table 1) replacing dietary protein by cassava leaf meal. The diets were offered ad libitum on plastic plates.

Table 1. Ingredients and chemical composition of diets (% as DM)

0CM

15CM

20CM

25CM

30CM

35CM

40CM

45CM

Rice bran

34

30

27

23

16

17

15

15

Maize

28

20

19

18

20

15

13

9

Soybean meal

21

16

14

14

13

12

10

9

Fish meal

16

15

15

14

14

13

13

12

Cassava leaf meal

0

18

24

30

36

42

48

54

Premix mineral-vitamin

1

1

1

1

1

1

1

1

Chemical compositions , % of DM

Crude protein

22.0

22.0

22.0

22.0

22.0

22.1

22.1

22.1

Ether extract

12.1

11.0

10.5

10.1

9.3

9.3

8.8

8.7

Crude fiber

7.7

9.4

9.8

10.3

10.4

11.2

11.7

12.5

The crickets were confined in plastic mesh cages (Photo 4) with dimensions of 50 cm x 30 cm x 20 cm). Cardboard egg holders (Photo 3) provide hiding and mounting places for the crickets. The crickets in each cage were weighed together at the start of the experiment and then after every week, before feeding in the morning, using an electronic scale. The amounts of feed offered and refused were recorded. Feed conversion ratio was calculated by dividing the total DM consumption by the total weight gain.

Photo 1. Cassava leaves, petioles and fine stems Photo 2. Cassava leaf meal


Photo 3. Arragement of egg trays Photo 4. The cages housing the crickets
Chemical analysis

Samples of ingredients and of diets offered and refused were analysed for DM, CP, CF, EE and ash according to AOAC (1990).

Statistical analysis

The data from the experiment were subjected to analysis of variance using the General Linear Model (GLM) procedure of Minitab Software Release version 17 (2013). Sources of variation were treatments and error. Response curves, relating outputs (weight gain, feed conversion and body composition of the crickets) with inputs (percentage of cassava leaf meal in the diet) were determined by fitting polynomial functions (Microsoft Excel Version 10) to the data.


Results and discussion

There were curvilinear relationships between levels of cassava leaf meal in the diet and the responses in DM intake, liveweight gain and feed conversion (Table 2 and 3; Figures 1-3). The feed intake continued to increase up to the highest level of intake of cassava leaf meal, but at a reduced rate. In contrast, the response in weight gain and feet conversion showed negative trends as the level of costs cassava leaf meal in the diet was increased beyond 20%.

Table 2. Feed intake during the experiment, mg/cricket/d

Item

0CM

15CM

20CM

25CM

30CM

35CM

40CM

45CM

SEM

p

Dry matter

37.7b

47.6ab

48.2ab

47.9ab

49.8a

51.0a

49.9a

53.8a

2.58

0.007

Crude protein

8.30b

10.5ab

10.6ab

10.6ab

11.0a

11.3a

11.1a

11.9a

0.57

0.006

Organic matter

34.6b

43.3ab

43.7ab

43.5ab

45.3a

46.2a

45.1ab

48.5a

2.34

0.011

Ether extract

4.56

5.25

5.03

4.85

4.64

4.75

4.41

4.70

0.25

0.353

Crude fiber

2.90d

4.52c

4.77bc

4.95bc

5.17bc

5.76abc

5.88ab

6.77a

0.29

0.000

abcd Means within columns with different superscripts are different at P<0.05



Table 3. Mean values for DM intake, live weight gain and feed conversion of crickets fed increasing proportions of cassava leaf meal

Item

0CM

15CM

20CM

25CM

30CM

35CM

40CM

45CM

SEM

p

DM intake, mg/d

37.7b

47.6ab

48.2ab

47.9ab

49.8a

51.0a

49.9a

53.8a

2.58

0.007

Initial weight, mg/cricket

3.1

3.1

3.1

3.0

3.2

3.1

3.2

3.2

0.08

0.75

Final weight, mg/cricket

802b

1001ab

1072a

984ab

969ab

980ab

932ab

939ab

44

0.01

ADG, mg/cricket/d

22.8b

28.5ab

30.5a

28.0ab

27.6ab

27.9ab

26.5ab

26.7ab

1.3

0.01

FCR, mgDM/mgADG

1.67bc

1.68bc

1.58c

1.70bc

1.80abc

1.83ab

1.88ab

2.01a

0.05

0.00

abc Means within columns with different superscripts are different at p<0.05



Figure 1. Effect of proportion of cassava leaf meal
in the diet on DM intake of crickets
Figure 2. Effect of proportion of cassava leaf meal
in the diet on growth of crickets

Figure 3. Effect of proportion of cassava leaf meal in the
diet on feed conversion ratio of crickets

The levels of crude protein and minerals in the crickets were increased with curvilinear tends as the content of cassava leaf meal in the diet was increased (Table 4, Figure 4 and 6). In contrast, the proportion of lipid on the cricket was reduced as the proportion of cassava leaf meals in the diet was increased (Table 4; Figure 5).

Table 4. Mean values for chemical composition of the cricket’s (% in DM)

Item

0CM

15CM

20CM

25CM

30CM

35CM

40CM

45CM

SEM

p

Dry matter

68.8

69.3

69.9

68.4

69.0

69.5

69.9

71.4

0.84

0.34

% in DM  

Crude protein

54.2g

55.0f

59.5cd

58.2e

58.8de

59.8c

61.6b

65.9a

0.18

<0.01

Ether extract

30.9a

29.9b

30.1b

28.1c

28.1c

26.3d

23.4e

21.8f

0.10

<0.01

Ash

3.49c

4.06b

4.66a

3.74c

4.15b

4.85a

4.30b

4.88a

0.07

<0.01

abcdefg Means within columns with different superscripts are different at p<0.05



Figure 4. Effect of level of cassava leaf meal in the diet
on crude protein content of crickets
Figure 5. Effect of level of dietary cassava leaf meal on
the ether extract content in the crickets

Figure 6. Effect of level of cassava leaf meal in the
diet on the ash content of crickets


Conclusions

There were curvilinear relationships between levels of cassava leaf meal in the diet and the responses in DM intake, liveweight gain and feed conversion. The feed intake continued to increase up to the highest level of intake of cassava leaf meal. However, the responses in weight gain and feed conversion reached maxim values with 20% of cassava leaf meal in the diet followed by negative responses beyond this level.

The protein and ash content of the crickets were increased with curvilinear trends as the of cassava leaf meal in the diet was increased. By contrast, the lipid content of the crickets was decreased by cassava leaf supplementation.


Acknowledgements

The authors are grateful for the financial for this research from Vietnam National University Ho Chi Minh City (VNU-HCM) under grand number C 2021-16-01/HĐ-KHCN.


References

Adedokun O O, Ewa E U, Eburuaja A S, Akinsola K L, Oketoobo E A, Ojewola G S and Ahamefule F O 2021 Anti-nutrients, Haematology, Serum Chemistry and Digestibility of Broiler Chickens Fed Graded Levels of Cassava Leaf meal Meal. Nigerian Journal of Animal Science and Technology (NJAST), 4(2), pp.76-85.

Angie S 2019 Survey Reveals Our Appetite for Eating Insects. Available online at: https://www.newshub.co.nz/home/rural/2019/07/survey-revealsour-appetite-for-eating-insects.html.

Anya M I, Ozung P O and Eburu P O 2019 Rumen degradation of cassava leaf meal meal supplemented with graded levels of monensin by N’Dama bulls in the humid zone of Nigeria.

AOAC 1990 Official Methods of Analysis, 15th edition. Association of the Official Analytical Chemists. Washington D.C.

Ayieko M A, Ogola H J and Ayieko I A 2016 Introducing rearing crickets (gryllids) at household levels: adoption, processing and nutritional values. JIFF. 2:203–11. doi: 10.3920/JIFF2015.0080.

Bani G 1995 Some aspects of entomophagy in the Congo. Food Insects Newsl.8:4–5.

Bodenheimer F S 1951 Insects as human food. In: Insects as Human Food. Springer: Dordrecht. p. 7–38. doi: 10.1007/978-94-017-6159-8_1.

Bui Phan Thu Hang, Nguyen Van Cop and Vo Lam 2020 Effect of cassava leaves (Manihot esculenta Crantz), water spinach (Ipomoea aquatica) and Coccinia grandis L. on biomass growth of crickets (Gryllus bimaculatus) fed chicken feed as the basal diet. Livestock Research for Rural Development, 32, Article #28, from http://www.lrrd.org/lrrd32/2/bpthan32028.html.

EFSA Scientific Committee 2015 Risk profile related to production and consumption of insects as food and feed. EFSA J. 13:4257. doi: 10.2903/j.efsa.2015.4257.

Fasae O A, Adu I F, Aina A B J, and Dipeolu M A 2011 Growth performance, carcass characteristics and meat sensory evaluation of west african dwarf sheep fed varying levels of maize and cassava hay. Trop. Anim. Health Prod. 43:503–510.

Feng Y, Chen XM, Zhao M, He Z., Sun L, Wang C Y and Ding W F 2017 Edible insects in China: utilization and prospects. Insect Sci. 25:184–98. doi: 10.1111/1744-7917.12449.

Frigerio J, Agostinetto G, Sandionigi A, Mezzasalma V, Berterame N M, Casiraghi M, Labra M and Galimberti A 2020 The hidden ‘plant side’of insect novel foods: a DNA-based assessment. Food Res Int. 128:108751. doi: 10.1016/j.foodres.2019.108751.

Gelfand M 1971 Insects. In: Diet and Tradition in an African Culture. Edinburgh: E&S. Livingstone. pp.163–171.

Halloran A, Megido R C, Oloo J, Weigel T, Nsevolo P, Francis F 2018 Comparative aspects of cricket farming in Thailand, Cambodia, Lao People’s Democratic Republic, Democratic Republic of the Congo and Kenya. JIFF. 4:101–14. doi: 10.3920/JIFF2017.0016.

Instar Farming 2020 Farming Crickets for Food in the UK. Available online at: https://www.instarfarming.com/

Jongema Y 2017 List of Edible Insects of the World. Wageningen: Laboratory of Entomology,Wageningen University.

Li M, Zhou H, Xu T and Zi X 2019 Effect of cassava leaf meal on the performance, carcass characteristics and gastrointestinal tract development of geese. Poultry science, 98(5), pp.2133-2138.

Magara H J, Tanga C M, Ayieko M A, Hugel S, Mohamed S A, Khamis F M, Salifu D, Niassy S, Sevgan S, Fiaboe K K M, Roos N and Ekesi S 2019 Performance of newly described native edible cricket Scapsipedus icipe (Orthoptera: Gryllidae) on various diets of relevance for farming. J Econ Entomol. 112:653–64. doi: 10.1093/jee/toy397.

Miech P, Berggren Å, Lindberg J E, Chhay T, Khieu B and Jansson A 2016 Growth and survival of reared Cambodian field crickets ( Teleogryllus testaceus) fed weeds, agricultural and food industry by-products. JIFF. (2016) 2:285–92. doi: 10.3920/JIFF2016.0028.

Nguyen T H L, Ngoan L D, Bosch G, Verstegen M W A, and Hendriks W H 2012 Ileal and total tract apparent crude protein and amino acid digestibility of ensiled and dried cassava leaves and sweet potato vines in growing pigs. Anim. Feed Sci. Technol. 172:171–179.

Oni A O, Arigbede O M, Oni O O, and Onwuka C 2010 Effects of feeding different levels of dried cassava leaves ( Manihot esculenta, Crantz) based concentrates with Panicum maximum basal on the performance of growing West African Dwarf goats. Livest. Sci. 129:24–30.

Oonincx D G, Van Broekhoven S, Van Huis A and van Loon J J 2015 Feed conversion, survival and development, and composition of four insect species on diets composed of food by-products. PLoS ONE. 10:e0144601. doi: 10.1371/journal.pone.0144601.

Orinda M A 2018 Effects of housing and feed on growth and technical efficiency of production of Acheta domesticus (L) and Gryllus bimaculatus for sustainable commercial crickets production in the lake victoria region, kenya. (Doctoral dissertation, JOOST). Available online at: http://ir.jooust.ac.ke:8080/xmlui/handle/123456789/8852.

Séré A, Bougma A, Ouilly J T, Traoré M, Sangaré H, Lykke AM, Ouédraogo A, Gnankiné O and Bassolé I H N 2018 Traditional knowledge regarding edible insects in Burkina Faso. J Ethnobiol Ethnomed. 14:1. doi: 10.1186/s13002-018-0258-z.

Van Huis A and Tomberlin J K 2017 Insects as food and feed: from production to consumption. the Netherlands: Waningen Academic Press.

Van Huis A, Van Itterbeeck J, Klunder H, Mertens E, Halloran A, Muir G and Vantomme P 2013 Edible insects: Future Prospects for Food and Feed Security; Food and Agriculture Organization of the United Nations: Rome, Italy.